從無致病性實(shí)驗(yàn)小鼠組織中分離得到BALB/c小鼠AcceGen原代腦血管成纖維細(xì)胞。小鼠原代腦血管成纖維細(xì)胞在預(yù)涂明膠溶液的T25組織培養(yǎng)瓶中生長0.5小時(shí)，一般在AcceGen培養(yǎng)完全生長培養(yǎng)基中純化擴(kuò)增培養(yǎng)3-7天。裝運(yùn)前，細(xì)胞從瓶中分離出來，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細(xì)胞每毫升，并冷凍運(yùn)送。小鼠大腦原代血管成纖維細(xì)胞對(duì)細(xì)菌、酵母、真菌和支原體呈陰性。免疫熒光染色檢測細(xì)胞標(biāo)記物的表達(dá)，抗體為anti-FSP1/S100A4。在AcceGen規(guī)定的細(xì)胞培養(yǎng)條件下，細(xì)胞可以1:2的分裂比擴(kuò)增2-5代。不建議反復(fù)冷凍和解凍細(xì)胞。細(xì)胞培養(yǎng)的標(biāo)準(zhǔn)生化程序包括測定細(xì)胞間相互作用、RT-PCR、Western blotting、免疫沉淀、免疫熒光染色、流式細(xì)胞術(shù)或產(chǎn)生用于所需研究應(yīng)用的細(xì)胞衍生物。

BALB/c Mouse Primary Brain Vascular Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Brain Vascular Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Brain Vascular Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

聲明：產(chǎn)品僅科研使用

For research use only