從無致病性實驗小鼠組織中分離得到BALB/c小鼠心臟內(nèi)皮細胞。小鼠心臟內(nèi)皮細胞在涂有明膠基培養(yǎng)基的T25組織培養(yǎng)瓶中生長0.5小時，一般在AcceGen的完全生長培養(yǎng)基中純化擴增培養(yǎng)3-7天。裝運前，細胞從瓶中分離出來，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細胞每毫升，并冷凍運送。小鼠心臟內(nèi)皮細胞可用于細胞間黏附、遷移、血管形成或跨內(nèi)皮細胞阻力(TER)的測定。內(nèi)皮細胞培養(yǎng)的標準生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫熒光染色或免疫熒光流式細胞術(shù)或產(chǎn)生用于所需研究應用的細胞衍生物。

BALB/c Mouse Cardiac Endothelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Cardiac Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. Mouse Cardiac Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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