BALB/c小鼠原代真皮成纖維細胞來自AcceGen，從無致病性實驗小鼠組織中分離得到。小鼠原代真皮成纖維細胞在預涂明膠溶液的T25組織培養(yǎng)瓶中生長0.5小時，一般在AcceGen培養(yǎng)完全生長培養(yǎng)基中培養(yǎng)3-7天至細胞開始擴增，裝運前，細胞從瓶中分離出來，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細胞每毫升，并冷凍運送。小鼠原代真皮成纖維細胞對細菌、酵母菌、真菌和支原體呈陰性。免疫熒光染色檢測細胞標記物的表達，抗體為anti-FSP1/S100A4。在AcceGen規(guī)定的細胞培養(yǎng)條件下，細胞可按1:2的分裂比例擴增3-5代。不建議反復冷凍和解凍細胞。細胞培養(yǎng)的標準生化程序包括測定細胞間相互作用、RT-PCR、Western blotting、免疫沉淀、免疫熒光染色、流式細胞術或產(chǎn)生用于所需研究應用的細胞衍生物。

BALB/c Mouse Primary Dermal Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Dermal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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