從無(wú)致病性實(shí)驗(yàn)小鼠組織中分離得到BALB/c小鼠皮膚淋巴內(nèi)皮細(xì)胞。小鼠真皮淋巴內(nèi)皮細(xì)胞在預(yù)涂明膠溶液的T25組織培養(yǎng)瓶中生長(zhǎng)0.5小時(shí)，一般在AcceGen的完全生長(zhǎng)培養(yǎng)基中培養(yǎng)3-7天至細(xì)胞開(kāi)始擴(kuò)增。裝運(yùn)前，細(xì)胞從瓶中分離出來(lái)，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細(xì)胞每毫升，并冷凍運(yùn)送。小鼠真皮淋巴管內(nèi)皮細(xì)胞可用于細(xì)胞間粘附、遷移、血管形成或跨內(nèi)皮細(xì)胞抵抗(TER)的檢測(cè)。內(nèi)皮細(xì)胞培養(yǎng)的標(biāo)準(zhǔn)生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫熒光染色或免疫熒光流式細(xì)胞術(shù)或產(chǎn)生用于所需研究應(yīng)用的細(xì)胞衍生物。

BALB/c Mouse Dermal Lymphatic Endothelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Dermal Lymphatic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. Mouse Dermal Lymphatic Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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