小鼠AcceGen原代細(xì)胞從無致病性實驗小鼠組織中分離。小鼠細(xì)胞在預(yù)涂明膠溶液的T25組織培養(yǎng)瓶中生長0.5小時，一般在AcceGen細(xì)胞培養(yǎng)基中培養(yǎng)3-7天至擴增培養(yǎng)，裝運前，細(xì)胞從瓶中分離出來，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細(xì)胞每毫升，并冷凍運送。小鼠細(xì)胞對細(xì)菌、酵母菌、真菌和支原體呈陰性。不建議反復(fù)冷凍和解凍細(xì)胞。細(xì)胞培養(yǎng)的標(biāo)準(zhǔn)生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫熒光染色、流式細(xì)胞術(shù)或產(chǎn)生用于所需研究應(yīng)用的細(xì)胞衍生物。

Mouse Primary Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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