AcceGen C57BL/6 小鼠原代卵巢成纖維細胞分離自無特定病原體實驗小鼠的組織。將組織種到T25瓶讓細胞成對數(shù)爬開，一般在AcceGen完全生長培養(yǎng)基里培養(yǎng)3-7天可以長滿，隨后進行傳代擴增。細胞從培養(yǎng)瓶分離出來立即冷凍保存在凍存管中，每支凍存管的細胞濃度不低于 1x10^6/毫升，冷凍運送。

小鼠原代卵巢成纖維細胞經(jīng)檢測不含細菌、酵母菌、真菌及支原體。采用抗 FSP1/S100A4 抗體，通過免疫熒光染色法對細胞進行標志物表達檢測。在 AcceGen 公司指定的細胞培養(yǎng)條件下，細胞可按 1:2 的比例傳代擴增 3～5 代。不建議對細胞進行反復凍融。

適用于該細胞培養(yǎng)的標準生化實驗包括細胞間相互作用檢測、逆轉(zhuǎn)錄 - 聚合酶鏈反應（RT-PCR）、蛋白質(zhì)印跡法（Western blotting）、免疫共沉淀、免疫熒光染色、流式細胞術(shù)，或制備用于特定研究用途的細胞衍生物。

C57BL/6 Mouse Primary Ovarian Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Ovarian Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

培養(yǎng)基配方：

成纖維細胞培養(yǎng)基FGCM(P6001）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎培養(yǎng)基+5%DMSO+20%FBS（SD0200)

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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