正常人皮膚成纖維細(xì)胞（HDF）為建立無血清的人胚胎干細(xì)胞飼養(yǎng)層或作為研究創(chuàng)傷愈合、毒理學(xué)或基礎(chǔ)細(xì)胞生物學(xué)的模型提供了理想的細(xì)胞系統(tǒng)。成纖維細(xì)胞作為原代細(xì)胞被冷凍保存，以確保最高的活性和純度。我們的成纖維細(xì)胞在LIFib無血清培養(yǎng)基和LIFib S2培養(yǎng)基中都進(jìn)行了培養(yǎng)測試，以確保細(xì)胞在經(jīng)典血清補(bǔ)充培養(yǎng)基的速率實(shí)現(xiàn)最佳生長。細(xì)胞特征：HDFn作為原代細(xì)胞被冷凍保存。從新生兒包皮中分離細(xì)胞，在冷凍保存前在培養(yǎng)血管中擴(kuò)增一次。原代HDFa細(xì)胞作為冷凍保存。細(xì)胞從成人皮膚中分離出來，在冷凍保存前在培養(yǎng)血管中擴(kuò)增一次。將人肺成纖維細(xì)胞冷凍保存，將人肺成纖維細(xì)胞從人肺中分離出來，在凍存前在培養(yǎng)血管中擴(kuò)增兩次。成纖維細(xì)胞可以在無血清、無動物蛋白、無酚紅和無抗菌劑的條件下生長。成纖維細(xì)胞經(jīng)過廣泛的測試，以達(dá)到質(zhì)量標(biāo)準(zhǔn)并表現(xiàn)出最佳性能。成纖維細(xì)胞適合用作人胚胎干細(xì)胞的無氙或非氙培養(yǎng)的飼養(yǎng)層，以及其他需要飼養(yǎng)層的細(xì)胞培養(yǎng)應(yīng)用。

normal Human Dermal Fibroblasts (HDF) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our fibroblasts are quality tested in both LIFib Serum-Free Medium and LIFib S2 Medium to ensure optimal growth at rates equal to or greater than classical serum-supplemented medium. Cell Features: HDFn are cryopreserved as primary cells. Cells are isolated from neonatal human foreskin and expanded in culture vessels once before cryopreservation. HDFa are cryopreserved as primary cells. Cells are isolated from adult human skin and expanded in culture vessels once before cryopreservation. Human Lung Fibroblasts are cryopreserved as secondary cells cells are isolated from human lungs and expanded in culture vessels twice before cryopreservation. Fibroblasts can be grown in serum-free, animal protein-free, phenol red-free, and antimicrobial free conditions when cultured in FibroLife Serum-Free Medium. Fibroblasts are extensively tested to meet quality standards and exhibit optimal performance. Fibroblasts are suitable for use as feeder layers as xeno-free or non-xeno free cultures for human embryonic stem cells and other cell culture applications requiring feeder layers.

For research use only