椎間盤退變是腰痛的主要病理過程。腰痛的原因被認(rèn)為與遺傳、環(huán)境和心理社會因素有關(guān)。纖維環(huán)構(gòu)成椎間盤結(jié)構(gòu)的外周部分，由纖維軟骨和Ⅰ、Ⅱ型膠原組成。椎間盤細(xì)胞在其局部環(huán)境中受到生物物理和機(jī)械因素的影響。白細(xì)胞介素1β可刺激纖維環(huán)細(xì)胞產(chǎn)生與局部降解和炎癥過程有關(guān)的因子。雖然機(jī)械應(yīng)力是退化的重要調(diào)節(jié)因子，但其潛在的分子機(jī)制仍不清楚。人椎間盤細(xì)胞培養(yǎng)為研究椎間盤退變的細(xì)胞和分子事件、組織工程和椎間盤細(xì)胞治療提供了一種體外模型HAFC紊亂從AcceGen研究實(shí)驗(yàn)室分離出人類椎間盤纖維環(huán)。HAFC在原代或傳代培養(yǎng)基中進(jìn)行冷凍保存，每小瓶1毫升容量中含有>5×10^5個細(xì)胞。用免疫熒光法對HAFC進(jìn)行了鑒定。HAFC細(xì)胞對HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌呈陰性。在AcceGen研究實(shí)驗(yàn)室提供的條件下，HAFC可達(dá)15倍增。

Degeneration of the intervertebral disk is a major pathological process implicated in low back pain. The causes of low back pain have been suggested to involve genetic, environmental, and psychosocial factors. The anulus fibrosus makes up the peripheral portion of disk structure, composed of fibrocartilage and type I and II collagen. Cells of the intervertebral disc are influenced by both biophysical and mechanical factors in their local environment. The anulus fibrosus cells can be stimulated by interleukin 1 beta to produce factors implicated in local degradative and inflammatory processes. Although mechanical stress is an important modulator of the degeneration, the underlying molecular mechanism remains unclear. The human annulus firosus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.HAFC from AcceGen Research Laboratories are isolated from annulus fibrosus of human intervertibral disc. HAFC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HAFC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HAFC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAFC are guaranteed to further expand for 15 population doublings in the condition provided by AcceGen Research Laboratories.

For research use only