Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Ovarian fibroblasts stimulate human ovarian cancer cell invasion and expression of 72-kDa gelatinase A (MMP-2).HOF from AcceGen Research Laboratories are isolated from human ovary tissue. HOF are cryopreserved at primary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HOF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HOF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.

For research use only