肺泡上皮細(xì)胞(Pulmonary alveolar epithelial cells, PAEpiC)由肺泡I型和II型上皮細(xì)胞組成，占肺內(nèi)表面積的99%以上。I型細(xì)胞是大的鱗狀細(xì)胞，其薄的胞漿延伸覆蓋了95%的內(nèi)表面積>。它們含有水通道蛋白，并表現(xiàn)出任何哺乳動(dòng)物細(xì)胞類(lèi)型中最高的滲透性水滲透性。II型細(xì)胞占表面積的2-5%，產(chǎn)生、分泌和循環(huán)肺表面活性劑。目前公認(rèn)的假設(shè)是，II型細(xì)胞通過(guò)調(diào)節(jié)肺中活躍的Na+運(yùn)輸來(lái)維持肺液穩(wěn)態(tài)，而I型細(xì)胞是“惰性”細(xì)胞，只提供屏障功能，而不具有活性功能。最近的研究表明，I型細(xì)胞在調(diào)節(jié)離子和液體運(yùn)輸方面也很重要。來(lái)自AcceGen研究實(shí)驗(yàn)室的HPAEpiC是從人類(lèi)肺組織中分離出來(lái)的。HPAEpiC在原代培養(yǎng)時(shí)低溫保存并冷凍運(yùn)送。每個(gè)凍存管至少含有1×10^6的細(xì)胞，使用CK-18、19和vimentin免疫熒光法驗(yàn)證HPAEpiC。HPAEpiC細(xì)胞對(duì)HIV-1, HBV, HCV，支原體，細(xì)菌，酵母和真菌檢測(cè)呈陰性。HPAEpiC需要在AcceGen研究實(shí)驗(yàn)室提供的條件下進(jìn)行培養(yǎng)。然而，這種細(xì)胞不建議擴(kuò)大或長(zhǎng)期培養(yǎng)，因?yàn)榧?xì)胞傳代后立即分化為I型肺泡上皮細(xì)胞，而I型肺泡上皮細(xì)胞在培養(yǎng)過(guò)程中不增殖。

Pulmonary alveolar epithelial cells (PAEpiC) comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal surface area. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the surface area, produce, secrete, and recycle pulmonary surfactant. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na+ transport in the lungs, whereas Type I cells are "inert" cells that provide solely a barrier function, rather than having active functions. Recent study indicate that Type I cells are also important in regulating ion and fluid transport.HPAEpiC from AcceGen Research Laboratories are isolated from human lung tissue. HPAEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPAEpiC are characterized by immunofluorescent method with antibodies CK-18, -19, and vimentin. HPAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAEpiC are guaranteed to further culture at the conditions provided by AcceGen Research Laboratories. However, this cell type is not recommend for expanding or long term cultures since the cells would differentiate to become type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.

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