The E11 cell line has been cloned from the outgrowth of glomeruli which were isolated from H-2kb-tsA58 transgenic mice. The mice carry a temperature-sensitive variant of the SV40 large T antigen under control of the IFN-g-inducible H-2kb promoter. Cells proliferate at 33°C, and they differentiate at 37°C. At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes.The species was confirmed by Real-Time PCR.

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