Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and GFP. Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal(Note: this is pooled stable ell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells.

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