Human Colorectal Adenocarcinoma (LS 174T) cells are isolated from a Dukes type B colorectal adenocarcinoma tissue by by centrifugal force following enzymatic digestion. GFP-expressing Human Colorectal Adenocarcinoma (GFP-LS 174T) are selected from LS 174T cells after infected with lentiviruses expressing GFP with puromycin. The cells are shipped in a frozen vial with more than 5 x 105 cells/vial. The Universal Full Growth Medium are recommended to culture the cells and these cells have a minimum population doubling capacity > 20 when cultured following the detailed protocol described below.

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