據報道，人腎近曲小管細胞HK-2含有HPV-16 E6/E7基因的重組逆轉錄病毒載體pLXSN 16 E6/E7。盡管pLXSN 16 E6/E7對新霉素有抗性，但不能用G418分離轉導的克隆。該細胞系基于Southern和FISH分析可分離成單個細胞。通過PCR測定，E6/E7基因存在于HK-2基因組中。細胞保留分化良好的PTCs表型。堿性磷酸酶、γ谷氨酰轉肽酶、亮氨酸氨肽酶、酸性磷酸酶、細胞角蛋白、α3、β1整聯蛋白和纖連蛋白表達陽性。VIII因子相關抗原、6.19抗原和CALLA內肽酶表達為陰性。HK-2細胞保留著近端腎小管上皮細胞的功能特征，例如Na+依賴性/根皮苷敏感性糖轉運和腺苷酸環(huán)化酶對甲狀旁腺有響應，但對抗利尿激素不敏感。該細胞具有生成和儲存糖原的能力來證明。HK-2細胞貼壁生長，在甲基纖維素，軟瓊脂上不生長且不能懸浮生長。

組織來源： 腎，皮質/近曲小管

形態(tài)：上皮細胞樣，貼壁生長

培養(yǎng)基配方：

K-SFM（Invitrogen，17005-042）培養(yǎng)液包含兩個組份:基礎培養(yǎng)液1瓶（10724-011)

+生長因子2管（37000-015 ）。

備注2：按照K-SFM培養(yǎng)液說明書，需額外添加Gentamicin Solution。建議使用Gentamicin/

mphotericin Solution 500×（Gibco, R-015-10）。

完全培養(yǎng)基：KGM細胞培養(yǎng)基 （P8001）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎培養(yǎng)基+5%DMSO+20%FBS（SD0200)

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產品僅科研使用。

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