取材于男性， 在PEG脅迫下，將原代培養(yǎng)的人臍靜脈細(xì)胞與抗硫鳥嘌呤的A549克隆株融合，構(gòu)建了人臍靜脈細(xì)胞株, EA.hy926。 融合子在HAT培養(yǎng)基上生長并以八因子相關(guān)抗原篩選。 EA.hy926已經(jīng)過100次以上的群體倍增(PDLs)。 電鏡照片顯示W(wǎng)eibel-Palade小體的細(xì)胞質(zhì)分布以及組織特異性的細(xì)胞器，分化的內(nèi)皮細(xì)胞特性如血管生成，體內(nèi)平衡/血栓形成，血壓及炎癥反應(yīng)。

組織來源 :體細(xì)胞融合子

形態(tài):上皮細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0100)+G/A (SD0038)

DMEM完全培養(yǎng)基（貨號：MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=6407019; DOI=10.1073/pnas.80.12.3734

Edgell C.-J.S., McDonald C.C., Graham J.B.

Permanent cell line expressing human factor VIII-related antigen established by hybridization.

Proc. Natl. Acad. Sci. U.S.A. 80:3734-3737(1983)

PubMed=3130908; DOI=10.1182/blood.V71.6.1669.1669

Emeis J.J., Edgell C.-J.S.

Fibrinolytic properties of a human endothelial hybrid cell line (Ea.hy 926).

Blood 71:1669-1675(1988)

PubMed=7791520; DOI=10.1016/0024-3205(95)00227-W

Ahn K., Pan S., Beningo K., Hupe D.

A permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells.

Life Sci. 56:2331-2341(1995)

PubMed=12453433; DOI=10.1006/mvre.2002.2434

Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.

In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.

Microvasc. Res. 64:384-397(2002)

PubMed=16575254; DOI=10.1097/01.mbc.0000220237.99843.a1

Boerma M., Burton G.R., Wang J., Fink L.M., McGehee R.E. Jr., Hauer-Jensen M.

Comparative expression profiling in primary and immortalized endothelial cells: changes in gene expression in response to hydroxy methylglutaryl-coenzyme A reductase inhibition.

Blood Coagul. Fibrinolysis 17:173-180(2006)

PubMed=28199884; DOI=10.1016/j.biomaterials.2017.02.005

Pietsch J., Gass S., Nebuloni S., Echegoyen D., Riwaldt S., Baake C., Bauer J., Corydon T.J., Egli M., Infanger M., Grimm D.

Three-dimensional growth of human endothelial cells in an automated cell culture experiment container during the SpaceX CRS-8 ISS space mission -- The SPHEROIDS project.

Biomaterials 124:126-156(2017)

PubMed=30108515; DOI=10.3389/fphys.2018.01025

Li N., Wang C.-Z., Sun S.-J., Zhang C., Lu D.-Y., Chen Q., Long M.

Microgravity-induced alterations of inflammation-related mechanotransduction in endothelial cells on board SJ-10 satellite.

Front. Physiol. 9:1025-1025(2018)

PubMed=30977987; DOI=10.33594/000000071

Kruger M., Pietsch J., Bauer J., Kopp S., Carvalho D.T.O., Baatout S., Moreels M., Melnik D., Wehland M., Egli M., Jayashree S., Kobbero S.D., Corydon T.J., Nebuloni S., Gass S., Evert M., Infanger M., Grimm D.

Growth of endothelial cells in space and in simulated microgravity -- a comparison on the secretory level.

Cell. Physiol. Biochem. 52:1039-1060(2019)