取材于CFW小鼠種屬。可體內(nèi)成瘤，廣泛用于腫瘤化療實(shí)驗(yàn)。

組織來源：腹水瘤 

形態(tài)：圓形；半貼半懸

培養(yǎng)基配方：

RPMI 1640（MD0200） +10%FBS（SD0200）+1%GA(SD0038)

完全培養(yǎng)基：RPMI1640完全培養(yǎng)基 （MD0218）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS（SD0200）

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=13035452; DOI=10.1093/jnci/13.5.1299

Dunham L.J., Stewart H.L.

A survey of transplantable and transmissible animal tumors.

J. Natl. Cancer Inst. 13:1299-1377(1953)

PubMed=13823721

Foley G.E., Drolet B.P., McCarthy R.E., Goulet K.A., Dokos J.M., Filler D.A.

Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.

Cancer Res. 20:930-939(1960)

PubMed=14185313; DOI=10.1126/science.146.3641.241

Cell Culture Collection Committee

Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.

Science 146:241-243(1964)

PubMed=14221803

Foley G.E., Drolet B.P.

Loss of neoplastic properties in vitro. I. Observations with S-180 cell lines.

Cancer Res. 24:1461-1467(1964)

PubMed=366289

Abercrombie M., Turner A.A.

Contact reactions influencing cell locomotion of a mouse sarcoma in culture.

Med. Biol. 56:299-303(1978)

PubMed=1546443; DOI=10.1016/0165-2427(92)90107-2

Alfaro G., Lomeli C., Ocadiz R., Ortega V., Barrera R., Ramirez M., Nava G.

Immunologic and genetic characterization of S180, a cell line of murine origin capable of growing in different inbred strains of mice.

Vet. Immunol. Immunopathol. 30:385-398(1992)