這株細(xì)胞是正常輸尿管組織用SV40病毒轉(zhuǎn)染建株的。 反復(fù)用非洲綠猴腎細(xì)胞平板測(cè)試檢測(cè)傳染性SV40的生成，結(jié)果都呈陰性。 脅迫（如曝露于化學(xué)試劑）下可能激活病毒。 ATCC還有一株化學(xué)轉(zhuǎn)化的成瘤細(xì)胞株，MC-SV-HUC T2，目錄號(hào)為CRL-9519，經(jīng)無(wú)支原體/STR鑒定入庫(kù)。

組織來(lái)源:輸尿管，輸尿管上皮

形態(tài):上皮細(xì)胞樣，貼壁生長(zhǎng)

培養(yǎng)基配方：

90%F12K(MD0400)+10%胎牛血清(SD0200)+1%GA(SD0038)

完全培養(yǎng)基鏈接:F12K完全培養(yǎng)基(MD0401)

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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Cancer Res. 48:3215-3220(1988)

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Reznikoff C.A., Christian B.J.

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