266-6是Robert E.Hammer于1985年用一只成年小鼠腺泡胰腺建系。用彈性蛋白酶I/SV-40t抗原融合基因誘導(dǎo)腫瘤細(xì)胞。該受體表達(dá)乙酰膽堿，毒蕈堿。這些細(xì)胞保持部分分化表型，并表達(dá)可檢測(cè)水平的消化酶mRNAs。細(xì)胞對(duì)氨甲膽堿和膽囊收縮素有反應(yīng)，但對(duì)P物質(zhì)、分泌素或血管活性腸肽（VIP）無反應(yīng)。細(xì)胞攜帶彈性蛋白酶/新霉素轉(zhuǎn)基因。

組織來源： 胰腺，胰腺腺泡癌

形態(tài)：上皮細(xì)胞樣，貼壁生長(zhǎng)

培養(yǎng)基配方：

90%DMEM（MD0100)+10%FBS(SD0200)+14mM HEPES(SD0009)+1%GA(SD0038)

完全培養(yǎng)基：DMEM完全培養(yǎng)基（MD0127）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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