細(xì)胞取樣于 BALB/cAnN種屬建系，鼠痘病毒測(cè)試呈陰性，細(xì)胞在Click培養(yǎng)基中表達(dá)較少的表面免疫球蛋白;在RPMI 1640培養(yǎng)基中表達(dá)量最大。細(xì)胞可以同時(shí)呈現(xiàn)同種異體抗原和蛋白質(zhì)抗原。經(jīng)無支原體鑒定入庫。

組織來源：細(xì)胞淋巴瘤細(xì)胞

形態(tài)：淋巴樣，懸浮生長(zhǎng)

培養(yǎng)基配方：

1640基礎(chǔ)（MD0200) +10%胎牛血清(SD0200）+ 0.05 mM巰基乙醇（SD0031）+1%GA(SD0038)

完全培養(yǎng)基：1640完全培養(yǎng)基（MD0201)

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=310843

Kim K.J., Kanellopoulos-Langevin C., Merwin R.M., Sachs D.H., Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843

Glimcher LH, et al. Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells. J. Exp. Med. 155: 445-459, 1982. PubMed: 6460073

Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306

Mallick-Wood CA, et al. Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase. Proc. Natl. Acad. Sci. USA 93: 9704-9709, 1996. PubMed: 8790395

Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998

Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022

Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843

Asofsky R.

Establishment and characterization of BALB/c lymphoma lines with B cell properties.

J. Immunol. 122:549-554(1979)

PubMed=22400032; DOI=10.1155/2012/701704

Donnou S., Galand C., Touitou V., Sautes-Fridman C., Fabry Z., Fisson S.

Murine models of B-cell lymphomas: promising tools for designing cancer therapies.

Adv. Hematol. 2012:701704-701704(2012)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)