膠質(zhì)細胞株C6是由Benda等用N-nitrosomethylurea誘導(dǎo)的大鼠膠質(zhì)瘤克隆，并經(jīng)過一系列的體外培養(yǎng)和動物傳代交替后建成的。 當細胞從低密度生長到滿瓶時，S-100產(chǎn)量增加10倍。經(jīng)無支原體鑒定入庫。

組織來源：膠質(zhì)瘤，腦，膠質(zhì)細胞

形態(tài)：成纖維細胞樣,貼壁生長

培養(yǎng)基配方：

87.5%F12K（MD0400）+10%胎牛血清（SD0200）+2.5%HS馬血清（SD0300)+1%GA(SD0038)

完全培養(yǎng)基：F12K完全培養(yǎng)基（MD0410） 

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS（SD0100)

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

CLPUB00233

Lightbody J.

Establishment of differentiated clonal strains of glial brain cells in culture.

Fed. Proc. 27:720-720(1968)

PubMed=4873531; DOI=10.1126/science.161.3839.370

Benda P., Lightbody J., Sato G., Levine L., Sweet W.

Differentiated rat glial cell strain in tissue culture.

Science 161:370-371(1968)

PubMed=3468959

Yoshida T., Shimizu K., Ushio Y., Mogami H., Sakamoto Y.

Possibility of overcoming of ACNU resistance in an ACNU-resistant subline of C6 rat glioma.

No To Shinkei 38:1065-1070(1986)

PubMed=19381449; DOI=10.1007/s11060-009-9875-7

Barth R.F., Kaur B.

Rat brain tumor models in experimental neuro-oncology: the C6, 9L, T9, RG2, F98, BT4C, RT-2 and CNS-1 gliomas.

J. Neurooncol. 94:299-312(2009)

PubMed=25492533; DOI=10.3109/19401736.2014.982619

Gao J., Hu L., Peng H.-H., Shi J.-G.

The complete mitochondrial genome sequence of the rat C6 glioma cell line.

Mitochondrial DNA 27:2188-2189(2016)