該細(xì)胞系源自于一名19歲車(chē)禍罹難的健康男性的視網(wǎng)膜組織，由AmyAotaki-Keen建系于1986年。該細(xì)胞系表達(dá)視網(wǎng)膜色素細(xì)胞特有的分子標(biāo)記如胞內(nèi)視黃醛結(jié)合蛋白和PRE-65。經(jīng)無(wú)支原體/STR鑒定入庫(kù)。

組織來(lái)源：眼睛；視網(wǎng)膜色素上皮；視網(wǎng)膜

形態(tài)：上皮細(xì)胞樣，貼壁生長(zhǎng)

培養(yǎng)基配方：

90%DMEM高糖（MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培養(yǎng)基（MD0118）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS　

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=8698076; DOI=10.1006/exer.1996.0020

Dunn K.C., Aotaki-Keen A.E., Putkey F.R., Hjelmeland L.M.

ARPE-19, a human retinal pigment epithelial cell line with differentiated properties.

Exp. Eye Res. 62:155-169(1996)

PubMed=11504951; DOI=10.1073/pnas.171266298

Lund R.D., Adamson P., Sauve Y., Keegan D.J., Girman S.V., Wang S., Winton H.L., Kanuga N., Kwan A.S.L., Beauchene L., Zerbib A., Hetherington L., Couraud P.-O., Coffey P., Greenwood J.

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.

Proc. Natl. Acad. Sci. U.S.A. 98:9942-9947(2001)

PubMed=14551534

Rogojina A.T., Orr W.E., Song B.K., Geisert E.E. Jr.

Comparing the use of Affymetrix to spotted oligonucleotide microarrays using two retinal pigment epithelium cell lines.

Mol. Vis. 9:482-496(2003)

PubMed=16262907; DOI=10.1186/1471-2415-5-25

Sharma R.K., Orr W.E., Schmitt A.D., Johnson D.A.

A functional profile of gene expression in ARPE-19 cells.

BMC Ophthalmol. 5:25-25(2005)

PubMed=21697133; DOI=10.1167/iovs.11-7479

Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.

Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011)

DOI=10.5897/IJBMBR2013.0154

Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.

Cell growth curves for different cell lines and their relationship with biological activities.

Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=24251032; DOI=10.1155/2013/216359

Pasovic L., Utheim T.P., Maria R., Lyberg T., Messelt E.B., Aabel P., Chen D.F., Chen X., Eidet J.R.

Optimization of storage temperature for cultured ARPE-19 cells.

J. Ophthalmol. 2013:216359-216359(2013)

PubMed=25177495; DOI=10.1155/2014/801787

Kuznetsova A.V., Kurinov A.M., Aleksandrova M.A.

Cell models to study regulation of cell transformation in pathologies of retinal pigment epithelium.

J. Ophthalmol. 2014:801787-801787(2014)

PubMed=27499609

Wang Y., Sang A.-M., Zhu M.-H., Zhang G.-W., Guan H.-J., Ji M., Chen H.

Tissue factor induces VEGF expression via activation of the Wnt/beta-catenin signaling pathway in ARPE-19 cells.

Mol. Vis. 22:886-897(2016)

PubMed=28978645; DOI=10.1074/jbc.M117.812677

Chiang C.-K., Tworak A., Kevany B.M., Xu B., Mayne J., Ning Z., Figeys D., Palczewski K.

Quantitative phosphoproteomics reveals involvement of multiple signaling pathways in early phagocytosis by the retinal pigmented epithelium.

J. Biol. Chem. 292:19826-19839(2017)

PubMed=29476476; DOI=10.1007/978-1-4939-7680-5_17

Fasler-Kan E., Aliu N., Wunderlich K., Ketterer S., Ruggiero S., Berger S., Meyer P.

The retinal pigment epithelial cell line (ARPE-19) displays mosaic structural chromosomal aberrations.

Methods Mol. Biol. 1745:305-314(2018)

PubMed=31254428; DOI=10.1111/ceo.13578

Churm R., Dunseath G.J., Prior S.L., Thomas R.L., Banerjee S., Owens D.R.

Development and characterization of an in vitro system of the human retina using cultured cell lines.

Clin. Exp. Ophthalmol. 47:1055-1062(2019)

PubMed=31412689; DOI=10.3390/pharmaceutics11080412

Hellinen L., Pirskanen L., Tengvall-Unadike U., Urtti A., Reinisalo M.

Retinal pigment epithelial cell line with fast differentiation and improved barrier properties.

Pharmaceutics 11:412.1-412.12(2019)