一位54歲白人男性的正常前列腺組織切片的周圍區(qū)域的上皮細(xì)胞用單拷貝的人乳頭瘤病毒18（HPV-18）進(jìn)行轉(zhuǎn)化，建立了RWPE-1。據(jù)報(bào)道，RWPE-1細(xì)胞在三維Matrigel培養(yǎng)時(shí)，在雄激素刺激下，形成腺胞（acini）并向培養(yǎng)基中分泌PSA。當(dāng)與Matrigel或基質(zhì)細(xì)胞混合注射雄性裸鼠時(shí)，RWPE-1細(xì)胞也能形成腺胞并產(chǎn)生PSA。來源于RWPE-1的細(xì)胞用Kirstin鼠肉瘤病毒（Ki-MuSV）轉(zhuǎn)染Ki-ras基因，建立了能成瘤的RWPE-2細(xì)胞和 RWPE2-W99細(xì)胞。另外，用N-甲醇-N-硝基脲（MNU）處理RWPE-1，建立了一系列模擬前列腺癌進(jìn)程中不同時(shí)期的成瘤細(xì)胞株。它們分別是WPE1-NA22, WPE1-NB14, WPE1-NB11和 WPE1-NB26細(xì)胞。據(jù)報(bào)道，RWPE-1細(xì)胞經(jīng)過檢測(cè)后發(fā)現(xiàn)乙肝病毒、丙肝病毒和人免疫缺陷病毒都呈陰性。PCR證實(shí)該細(xì)胞系HPV病毒DNA序列呈陽性。經(jīng)無支原體/STR鑒定入庫。

組織來源： 前列腺

形態(tài)：上皮細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

KGM角質(zhì)細(xì)胞無血清培養(yǎng)基（P8001）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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