源于C57BL/6小鼠小膠質(zhì)細胞，表達核v-myc、染色體v-raf癌基因，表面表達envgp70抗原，在形態(tài)學(xué)、表型及功能上有吞噬細胞的特征。 經(jīng)無支原體鑒定入庫。

組織來源：小膠質(zhì)細胞

形態(tài)：半貼壁生長

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：90%FBS(SD0200)+10%DMSO

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=2110186; DOI=10.1016/0165-5728(90)90073-V

Blasi E., Barluzzi R., Bocchini V., Mazzolla R., Bistoni F.

Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.

J. Neuroimmunol. 27:229-237(1990)

PubMed=19565166

Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.

The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.

ALTEX 26:83-94(2009)

PubMed=21961028; DOI=10.2174/1877381801002010148

Crain J.M., Watters J.J.

Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.

Open Drug Discov. J. 2:148-167(2010)

PubMed=21214938; DOI=10.1186/1471-2164-12-14

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14-14(2011)

PubMed=22651808; DOI=10.1186/1742-2094-9-115

Stansley B., Post J., Hensley K.

A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.

J. Neuroinflamm. 9:115-115(2012)

PubMed=26227174; DOI=10.1002/pmic.201400623

Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

DOI=10.1007/s13273-019-00057-6

Mandal C., Yoon T., Park J.Y., Jung K.H., Chai Y.G.

Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells.

Mol. Cell. Toxicol. 16:51-61(2020)