該細(xì)胞系建立于C57BL小鼠的肺，可能與3LL細(xì)胞是同株細(xì)胞，該小鼠的腫瘤源于原發(fā)性Lewis肺癌的植入。細(xì)胞對1,3-雙(2-氯乙基)-1-亞硝基脲耐藥，但對甲氨蝶呤敏感。據(jù)報道，這些細(xì)胞在小鼠中具有高度的致瘤性，但轉(zhuǎn)移性較弱。細(xì)胞在燒瓶中形成多層，沒有真正融合。經(jīng)測試，鼠痘病毒呈陰性。 經(jīng)無支原體鑒定入庫。

組織來源：肺

形態(tài)：混合形態(tài)，半貼壁生長

培養(yǎng)基配方:

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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