Neuro-2A是由R.J. Klebe和F.H. Ruddle經(jīng)A品系白鼠的自發(fā)腫瘤建立。取材于A品系小鼠，這個(gè)腫瘤小鼠C1300購自Jackson Laboratory。該細(xì)胞產(chǎn)生大量微管蛋白，此蛋白在神經(jīng)細(xì)胞中起應(yīng)答軸漿流動(dòng)的收縮系統(tǒng)起作用。檢測小鼠痘病毒為陰性。經(jīng)無支原體鑒定入庫。

組織來源：腦 

形態(tài)：神經(jīng)元和阿米巴樣干細(xì)胞，貼壁生長

培養(yǎng)基配方：

90%MEM（MD0300）+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（MD0301）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=11470893; DOI=10.1073/pnas.151242598

Enari M., Flechsig E., Weissmann C.

Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.

Proc. Natl. Acad. Sci. U.S.A. 98:9295-9299(2001)

PubMed=14504404; DOI=10.1073/pnas.1834432100

Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.

A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.

Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)

PubMed=19903493; DOI=10.1016/j.jneumeth.2009.11.004

Tremblay R.G., Sikorska M., Sandhu J.K., Lanthier P., Ribecco-Lutkiewicz M., Bani-Yaghoub M.

Differentiation of mouse Neuro 2A cells into dopamine neurons.

J. Neurosci. Methods 186:60-67(2010)

PubMed=25193168; DOI=10.1074/mcp.M113.037291

Hornburg D., Drepper C., Butter F., Meissner F., Sendtner M., Mann M.

Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

Mol. Cell. Proteomics 13:3410-3420(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)