DLD-1 是1977-1979年間D.L. Dexter和同事分離的兩株結(jié)直腸腺癌細(xì)胞株中的一株。 在ATCC和其它地方進(jìn)行的DNA fingerprinting和染色體組型分析表明這株細(xì)胞與HCT-15 (CCL-225)相似，說明這兩者是來自同一個(gè)人的不同克隆。 他們的遺傳起源可通過DNA fingerprinting證實(shí)，但染色體組型分析顯示它們?nèi)狈θ旧w標(biāo)記一致改變或數(shù)目上一致改變。 細(xì)胞的CSAp陰性(CSAp-)。 DLD-1 細(xì)胞的 p53 抗原表達(dá)呈陽性(p53 抗原產(chǎn)生了一個(gè)C -> T點(diǎn)突變導(dǎo)致241位的Ser -> Phe)。 角蛋白免疫過氧化物酶染色陽性。 癌基因c-myc, K-ras, H-ras, N-ras, myb, sis 和fos的表達(dá)呈陽性。 癌基因N-myc的表達(dá)未做檢測。 表達(dá)腫瘤特異性核基質(zhì)蛋白CC-2, CC-3, CC-4, CC-5 和 CC-6。 1979年提交到ATCC的培養(yǎng)物代數(shù)不明且污染了支原體。 其后經(jīng)過12周多種抗生素聯(lián)合培養(yǎng)處理。 處理之后每周用Hoechst染色和標(biāo)準(zhǔn)培養(yǎng)法檢測。 其后連續(xù)11個(gè)月不加抗生素培養(yǎng)，所有的檢測呈陰性。

組織來源：結(jié)直腸腺癌

形態(tài)：上皮細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

90%DMEM高糖(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：DMEM高糖完全培養(yǎng)基（MD0101）

氣相：空氣，100%。 溫度：37℃

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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