C166細(xì)胞系是由雌性NMRI/GSF小鼠與雄性CD-1小鼠交配獲得的F1胚胎細(xì)胞建立的，該小鼠是轉(zhuǎn)人fes (fps/fes)原癌基因。該細(xì)胞系可用于研究?jī)?nèi)皮細(xì)胞分化和確定建立器官特異性?xún)?nèi)皮細(xì)胞異質(zhì)性的機(jī)制，并可支持多能造血干細(xì)胞的穩(wěn)定增殖，從而產(chǎn)生足夠數(shù)量的細(xì)胞，用于研究其后續(xù)發(fā)育和分化的機(jī)制。

種屬：小鼠

組織來(lái)源：血管內(nèi)皮

形態(tài)：貼壁生長(zhǎng) 上皮樣

培養(yǎng)基配方：

DMEM(高糖)(MD0100)+10%FBS(SD0200)+1%G/A（SD0038）

完全培養(yǎng)基：DMEM高糖培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

聲明：產(chǎn)品僅科研使用。

Wang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159

Lu LS, et al. In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor cells expanded > 100-fold by coculture with a clonal yolk sac endothelial cell line. Proc. Natl. Acad. Sci. USA 93: 14782-14787, 1996. PubMed: 8962132

Wang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159