細胞來源于13周C57BL/6小鼠，MIN6細胞分泌胰島素、胰高血糖素、生長抑素和ghrelin。

組織來源：小鼠胰島

形態(tài)：梭形、多角形，貼壁生長

培養(yǎng)基配方：

90%1640(MD0200)+10%胎牛血清(SD0200)+β-Mer 0.05mM（SD0031）+1%G/A (SD0038)

完全培養(yǎng)基：1640完全培養(yǎng)基（MD0206）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS　

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=2163307; DOI=10.1210/endo-127-1-126

Miyazaki J.-I., Araki K., Yamato E., Ikegami H., Asano T., Shibasaki Y., Oka Y., Yamamura K.-I.

Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms.

Endocrinology 127:126-132(1990)

PubMed=18845907; DOI=10.1507/endocrj.K08E-172

Nakashima K., Kanda Y., Hirokawa Y., Kawasaki F., Matsuki M., Kaku K.

MIN6 is not a pure beta cell line but a mixed cell line with other pancreatic endocrine hormones.

Endocr. J. 56:45-53(2009)

PubMed=22538498; DOI=10.1159/000338793

Grozinsky-Glasberg S., Shimon I., Rubinfeld H.

The role of cell lines in the study of neuroendocrine tumors.

Neuroendocrinology 96:173-187(2012)

PubMed=22808281; DOI=10.1371/journal.pone.0040868

Cheng K., Delghingaro-Augusto V., Nolan C.J., Turner N., Hallahan N., Andrikopoulos S., Gunton J.E.

High passage MIN6 cells have impaired insulin secretion with impaired glucose and lipid oxidation.

PLoS ONE 7:E40868-E40868(2012)

PubMed=25546123; DOI=10.1002/pmic.201400345

Lee J.-S., Wu Y., Schnepp P., Fang J., Zhang X., Karnovsky A., Woods J., Stemmer P.M., Liu M., Zhang K., Chen X.

Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells.

Proteomics 15:1508-1511(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314

Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.

A mass spectrometric-derived cell surface protein atlas.

PLoS ONE 10:E0121314-E0121314(2015)