HET-1A細胞系是1986年從人食管尸檢組織中通過轉(zhuǎn)染質(zhì)粒pRSV-T獲得的，該質(zhì)粒由RSV-LTR啟動子和編碼猿猴病毒40大T抗原的序列組成。細胞已經(jīng)過超250多次的傳代倍增，生長因子研究表明，HET-1A受到鈣的刺激，并受到胎牛血清、轉(zhuǎn)化生長因子β1和轉(zhuǎn)化生長因子β2的抑制。伏馬菌素B1在HET-1A細胞中產(chǎn)生生長抑制和凋亡增加。合成維甲酸CD437（6-[3-（1-金剛基）-4-羥基苯基]-2-萘甲酸（AHPN/CD437）0通過caspase-3依賴途徑誘導(dǎo)食管鱗狀上皮HET-1A細胞凋亡，該細胞系可能有助于研究假定的食管致癌物的作用。

組織來源：食管

形態(tài) ：上皮細胞樣，貼壁生長

培養(yǎng)基配方：

TraEp 氣道上皮細胞培養(yǎng)基 (P1601)

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：95%FBS+5%DMSO(SD0200)，程序降溫

完全凍存液：低血清免程序細胞凍存液（SD0001） 

CLPUB00265

Stoner G.D., Kaighn M.E., Resau J.H., Naito Z., Bowman D., Harris C.C.

Immortalized human esophageal epithelial cell lines for studies of carcinogen-induced cell transformation.

In Vitro Cell. Dev. Biol. 26:23A-23A(1990)

DOI=10.1007/978-1-4612-0411-4_30

Stoner G.D., Light B.A., Harris C.C.

Immortalization and oncogene transformation of human esophageal epithelial cells.

(In) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.297-305; Humana Press; New York (1990)

Patent=WO1991005062A1

Stoner G.D., Reddel R.R., Harris C.C.

Human esophageal epithelial cell lines.

Patent number WO1991005062A1, 18-Apr-1991

PubMed=1703038

Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C.

Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells.

Cancer Res. 51:365-371(1991)

PubMed=8504475; DOI=10.1093/carcin/14.5.833

Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.

p53 mutations in human immortalized epithelial cell lines.

Carcinogenesis 14:833-839(1993)

PubMed=7972006; DOI=10.1073/pnas.91.23.11045

Okamoto A., Demetrick D.J., Spillare E.A., Hagiwara K., Hussain S.P., Bennett W.P., Forrester K., Gerwin B., Serrano M., Beach D.H., Harris C.C.

Mutations and altered expression of p16INK4 in human cancer.

Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994)

PubMed=10078940; DOI=10.1002/(sici)1098-2744(199902)24:2

Compton K.R., Orringer M.B., Beer D.G.

Induction of glutathione s-transferase-pi in Barrett's metaplasia and Barrett's adenocarcinoma cell lines.

Mol. Carcinog. 24:128-136(1999)

PubMed=20139130; DOI=10.1093/carcin/bgq036

Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P.

An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.

Carcinogenesis 31:936-945(2010)

PubMed=20843300; DOI=10.1042/BC20100071

Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P.

A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture.

Biol. Cell 102:635-644(2010)

PubMed=21127259; DOI=10.1152/ajpgi.00461.2010

Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K.

Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.

Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)

PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002

Akagi I., Ishibashi O.

RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium.

Genomics Data 12:4-6(2017)