人骨髓間充質(zhì)干細(xì)胞（HMSC-BM）為研究多潛能干細(xì)胞生物學(xué)和分化過程提供了理想的培養(yǎng)模型。從骨髓抽出物中分離、擴(kuò)增一次并在P1代純化后，P2擴(kuò)增，P3代凍存，以確保最佳表型、最高的活力和貼壁能力；該細(xì)胞在未分化的時(shí)可至少達(dá)15倍擴(kuò)增，人間充質(zhì)干細(xì)胞-骨髓可分化為典型的成脂肪細(xì)胞、成軟骨細(xì)胞和成骨細(xì)胞，經(jīng)CD29、CD44等免疫熒光檢測。

培養(yǎng)基配方：

間充質(zhì)干細(xì)胞完全培養(yǎng)基(貨號：P2001)

氣相：空氣，95%；二氧化碳，5%；溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

1. S. et al. The study on bone marrow mesenchymal stem cell-derived extracellular matrix promoting the repair of damaged chondrocytes by regulating the Notch1/RBPJ pathway. Cytotechnology 77, 35 (2025).

 https://doi.org/10.1007/s10616-024-00702-6

2. Hong Wang (2024)Fibroblast growth factor 2 enhances BMSC stemness through ITGA2-dependent PI3K/AKT pathway activation；

https://doi.org/10.1002/jcp.31423

3. Huang, J., Zeng, N., Xu, S. et al. The study on bone marrow mesenchymal stem cell-derived extracellular matrix promoting the repair of damaged chondrocytes by regulating the Notch1/RBPJ pathway. Cytotechnology 77, 35 (2025). 

https://doi.org/10.1007/s10616-024-00702-6