在20世紀90年代中期從Gl261腫瘤建立了體外生長細胞培養(yǎng)，該腫瘤于1939年通過顱內(nèi)注射3-甲基膽蒽到C57BL/6小鼠中誘導(dǎo)，隨后通過在同基因小鼠系中的連續(xù)移植維持；文獻中描述細胞攜帶TP53和KRAS突變。

組織來源：膠質(zhì)瘤

形態(tài)：成纖維細胞樣，貼壁生長

培養(yǎng)基配方:

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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