從一個(gè)巨核細(xì)胞白血病患者的血液建立了一株新的人類巨核細(xì)胞(Dami)。 此細(xì)胞懸浮生長(zhǎng)為主，倍增時(shí)間24-30小時(shí)。至少89%的細(xì)胞可與血小板糖蛋白(GP) lb and Ilb/llla及血型糖蛋白單克隆抗體反應(yīng)。不與抗淋巴腺、單核細(xì)胞、粒性白細(xì)胞、巨噬細(xì)胞抗原的抗體反應(yīng)。Dami細(xì)胞為研究巨核細(xì)胞生化及分化提供了極好的模型；Problematic cell line: Contaminated. Shown to be a HeL derivative 。具體STR位點(diǎn)信息如下：D5818 : 11 , 11 : D13S317 :9 , 11: D7S20 : 1,1: D1S59 : 1 , WA:147TH01 : 7 ，7 ; AMEL : XX; TPOX :11,11: CSFPO :10,10:

組織來源：巨核細(xì)胞白血病

形態(tài)：巨核細(xì)胞，懸浮為主、少量貼壁

培養(yǎng)基配方：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：1640完全培養(yǎng)基（MD0201）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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DAMI cell line.

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