LMH是一種原發(fā)性肝細(xì)胞癌上皮細(xì)胞系，由Tomoyuki Kitagawa于1981年在日本東京都島Kami-Ikebukuro的癌癥研究所建立。用二乙基亞硝胺長期治療長腿角雞，在其肝臟中誘發(fā)腫瘤結(jié)節(jié)。細(xì)胞的形態(tài)是樹狀的。細(xì)胞表達(dá)葡萄糖-6-磷酸酶和微弱的小管ATP酶活性。這些細(xì)胞在無胸腺裸鼠體內(nèi)形成腫瘤。LMH細(xì)胞含有低水平的功能性雌激素受體，并且可誘導(dǎo)肝臟特異性載脂蛋白II（apoII）基因的表達(dá)。該細(xì)胞系可用于轉(zhuǎn)染研究。

注：培養(yǎng)該細(xì)胞的培養(yǎng)耗材需要使用0.1%的明膠包被，或者使用基質(zhì)膠包被。

培養(yǎng)基配方：

90%DMEM/F12(MD0600)+10%胎牛血清(SD0200)+G/A (SD0038) 

完全培養(yǎng)基：DMEM/F12完全培養(yǎng)基（MD0601）

氣相：空氣，95%；二氧化碳，5%。 溫度：33.5℃

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=3607775

Kawaguchi T., Nomura K., Hirayama Y., Kitagawa T.

Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH.

Cancer Res. 47:4460-4464(1987)

PubMed=2330000; DOI=10.1210/mend-4-2-201

Binder R., MacDonald C.C., Burch J.B.E., Lazier C.B., Williams D.L.

Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line.

Mol. Endocrinol. 4:201-208(1990)

PubMed=2352324; DOI=10.1128/JVI.64.7.3249-3258.1990

Condreay L.D., Aldrich C.E., Coates L., Mason W.S., Wu T.-T.

Efficient duck hepatitis B virus production by an avian liver tumor cell line.

J. Virol. 64:3249-3258(1990)

PubMed=10564780; DOI=10.1016/s0304-4165(99)00159-2

Kolluri S., Elbirt K.K., Bonkovsky H.L.

Heme biosynthesis in a chicken hepatoma cell line (LMH): comparison with primary chick embryo liver cells (CELC).

Biochim. Biophys. Acta 1472:658-667(1999)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

Karger A., Bettin B., Lenk M., Mettenleiter T.C.

Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5

Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.

High-throughput sequencing for species authentication and contamination detection of 63 cell lines.

Sci. Rep. 11:21657-21657(2021)