中山醫(yī)院取材，由武漢同濟(jì)大學(xué)建系，用人肝癌細(xì)胞株MHCC97-H接種裸鼠，進(jìn)行3次肺轉(zhuǎn)移篩選，取肺轉(zhuǎn)移瘤細(xì)胞皮下接種后高度自發(fā)性肺轉(zhuǎn)移的肝癌細(xì)胞系。

動物種別：人

組織來源：肝臟

形態(tài)：上皮細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

DMEM高糖(MD0100)+10%FBS(SD0200)+1%G/A(SD0038)

完全培養(yǎng)基：DMEM高糖完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

聲明：產(chǎn)品僅科研使用。

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Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97.

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Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials.

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Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics.

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Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.

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Phosphoproteomic analysis of the highly-metastatic hepatocellular carcinoma cell line, MHCC97-H.

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PubMed=25886575; DOI=10.1186/s12885-015-1166-4

Dang H., Steinway S.N., Ding W., Rountree C.B.

Induction of tumor initiation is dependent on CD44s in c-Met(+) hepatocellular carcinoma.

BMC Cancer 15:161-161(2015)

PubMed=31063779; DOI=10.1053/j.gastro.2019.05.001

Caruso S., Calatayud A.-L., Pilet J., La Bella T., Rekik S., Imbeaud S., Letouze E., Meunier L., Bayard Q., Rohr-Udilova N., Peneau C., Grasl-Kraupp B., de Koning L., Ouine B., Bioulac-Sage P., Couchy G., Calderaro J., Nault J.-C., Zucman-Rossi J., Rebouissou S.

Analysis of liver cancer cell lines identifies agents with likely efficacy against hepatocellular carcinoma and markers of response.

Gastroenterology 157:760-776(2019)

PubMed=31378681; DOI=10.1016/j.ccell.2019.07.001

Qiu Z.-X., Li H., Zhang Z.-T., Zhu Z.-F., He S., Wang X.-J., Wang P.-C., Qin J.-J., Zhuang L.-P., Wang W., Xie F.-B., Gu Y., Zou K.-K., Li C., Li C., Wang C.-H., Cen J., Chen X.-T., Shu Y.-J., Zhang Z., Sun L.-L., Min L.-H., Fu Y., Huang X.-W., Lv H., Zhou H., Ji Y., Zhang Z.-G., Meng Z.-Q., Shi X.-L., Zhang H.-B., Li Y.-X., Hui L.-J.

A pharmacogenomic landscape in human liver cancers.

Cancer Cell 36:179-193.e11(2019)