CEM/C1是人T細(xì)胞白血病細(xì)胞株CCRF-CEM(見ATCCCCL-119)具有喜樹堿抗性的衍生株。1991年細(xì)胞株選擇并亞克隆了對CPT的抗性。細(xì)胞表現(xiàn)出對CPT類似物水溶性的托泊替康和非水溶性的9-氨基-CPT及10,11-亞甲二氧基-CPT具有交叉抗性。CEM/C1細(xì)胞對CPT的敏感性較母系CEM細(xì)胞低31倍。CEM/C1細(xì)胞表現(xiàn)非典型的多藥抗性和轉(zhuǎn)換拓補(bǔ)異構(gòu)酶I催化活性。對CPT的抗性維持6個月以上。經(jīng)無支原體/STR鑒定入庫。

動物種別：人

組織來源：外周血

形態(tài):淋巴母細(xì)胞樣， 懸浮生長

培養(yǎng)基配方：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：1640完全培養(yǎng)基（MD0201）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458

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A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)