1948年三月建立了NCTC clone 929(小鼠結(jié)締組織)，細(xì)胞系L的克隆。細(xì)胞系L是最早建立的連續(xù)培養(yǎng)細(xì)胞系之一，而clone 929是最早的克隆株。 從一只100日齡的雄性C3H/An小鼠的正常皮下疏松結(jié)締組織入脂肪組織中建立了親本細(xì)胞系L。 第95代的細(xì)胞系L使用毛細(xì)管法分離單細(xì)胞建立了clone929。經(jīng)無支原體鑒定入庫。

動物種別：小鼠

組織來源：組織：皮下結(jié)締組織；疏松結(jié)締組織及脂肪 品系：C3H/An

形態(tài)：成纖維細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：90%FBS(SD0200)+10%DMSO

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

Static Magnetic Fields Reduce Oxidative Stress to Improve Wound Healing and Alleviate Diabetic Complications

Cells 2022, 11(3), 443; https://doi.org/10.3390/cells11030443

Collagen from Iris squid grafted with polyethylene glycol and collagen peptides promote the proliferation of fibroblast through PI3K/AKT and Ras/RAF/MAPK signaling pathways，https://doi.org/10.1016/j.ijbiomac.2023.125772

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)

PubMed=32218446; DOI=10.1038/s41597-020-0449-z

Zhong C.-Q., Wu J.-F., Qiu X.-F., Chen X., Xie C.-C., Han J.-H.

Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins.Sci. Data 7:104-104(2020)

Injectable Living Probiotic Dressing Built by Droplet-Based Microfluidics And Photo-Crosslinking to Prevent Pathogenic Infection And Promote Wound Repair

published: 16 October 2023

 https://doi.org/10.1002/adhm.202302423