1975年從患有淋巴腫瘤的DBA/2小鼠腹水中建立了小鼠P-388D1單核/巨噬細(xì)胞細(xì)胞系(Koren et al. 1975)。P-388D1細(xì)胞為橢圓形、圓形、松散貼壁細(xì)胞，單層生長。在被脂多糖或富爾寶-12-肉豆蔻酸-13-醋酸酯刺激后，它們產(chǎn)生IL-1和LAF(淋巴細(xì)胞激活因子)。細(xì)胞吞噬酶和乳膠珠，并在抗體依賴的細(xì)胞介導(dǎo)的細(xì)胞毒性系統(tǒng)中活躍。
組織來源：淋巴瘤
形態(tài)：淋巴母細(xì)胞樣，懸浮生長

培養(yǎng)基配方：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：1640完全培養(yǎng)基（MD0201）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

CLPUB00459

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