WI-38人二倍體細(xì)胞系來自妊娠3個月的正常胚胎肺組織。 該細(xì)胞系是首株用于人類疫苗制備的人二倍體細(xì)胞系。 

培液中添加TNF alpha可以促進細(xì)胞生長。

組織來源：正常肺

形態(tài)：成纖維細(xì)胞樣,懸浮生長

培養(yǎng)基配方：

87%MEM(MD0300)+10%胎牛血清(SD0200)++ L-glutamine(SD0007)+NEAA+SP（SD0008）+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（MD0302）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

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