B16-Fo是一株小鼠黑絲素瘤細胞系。它可在體外常規(guī)傳代培養(yǎng)，產(chǎn)生高濃度的黑色素。可用于分子生物學(xué)中黑色素基因的表達研究。
形態(tài)：上皮樣細胞，貼壁生長

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=1109790

Fidler I.J.

Biological behavior of malignant melanoma cells correlated to their survival in vivo.

Cancer Res. 35:218-224(1975)

PubMed=11833741; DOI=10.1016/S0024-3205(01)01454-0

Nakamura K., Yoshikawa N., Yamaguchi Y., Kagota S., Shinozuka K., Kunitomo M.

Characterization of mouse melanoma cell lines by their mortal malignancy using an experimental metastatic model.

Life Sci. 70:791-798(2002)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)

PubMed=34755401； DOI=10.1002/chem.202103346

Zhongxian Fan,Jiaen Xie,Dr. Tumpa Sadhukhan,Chao Liang,Can Huang,Wenqing Li,Tingxuan Li,Dr. Pingyu Zhang,Dr. Samya Banerjee,Dr. Krishnan Raghavachari,Dr. Huaiyi Huang

Catalyst for Synergetic Multi-Mode Cancer Photo-Therapy（2021）