PK-15細(xì)胞建系于1955（Stice,E）。是PK-1a細(xì)胞的克隆系。該細(xì)胞系可用于多種病毒的增值及特性研究。另外，電鏡觀察發(fā)現(xiàn)，PK-15細(xì)胞內(nèi)有C-型病毒顆粒存在，是研究C-型病毒的材料。

形態(tài)：上皮細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=5962858

Pirtle E.C.

Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.

Am. J. Vet. Res. 27:747-749(1966)

PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X

Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.

Characterization of a type C virus released from the porcine cell line PK(15).

Virology 58:65-74(1974)

PubMed=4151202

Tischer I., Rasch R., Tochtermann G.

Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.

Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)

PubMed=3009166; DOI=10.1507/endocrj1954.32.819

Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A., Fujita T.

Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.

Endocrinol. Jpn. 32:819-828(1985)

PubMed=3173126

Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.

Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.

Microbiologica 11:263-264(1988)

PubMed=2686830

Dulac G.C., Afshar A.

Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.

Can. J. Vet. Res. 53:431-433(1989)

PubMed=17889922; DOI=10.1016/j.virol.2007.08.014

Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K., Kwang J.H.-S.

Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.

Virology 369:423-430(2007)

PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x

Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.

Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.

Int. J. Immunogenet. 36:231-234(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

Karger A., Bettin B., Lenk M., Mettenleiter T.C.

Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

PubMed=26456528; DOI=10.1126/science.aad1191

Yang L., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J., Shrock E., Xu W., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A., Church G.M.

Genome-wide inactivation of porcine endogenous retroviruses (PERVs).

Science 350:1101-1104(2015)