該細(xì)胞系是由轉(zhuǎn)基因小鼠大腦中產(chǎn)生的腫瘤培養(yǎng)物建立起來(lái)的細(xì)胞株，該轉(zhuǎn)基因包含與783bp部分酪氨酸羥化酶基因相關(guān)的SV40T抗原編碼序列,細(xì)胞表達(dá)酪氨酸羥化酶和多巴胺β羥化酶，并含有神經(jīng)絲。

動(dòng)物種別：小鼠

組織來(lái)源：神經(jīng)細(xì)胞

形態(tài)：上皮細(xì)胞樣；貼壁生長(zhǎng)

培養(yǎng)基配方：

87%RPMI1640(MD0200)+8%馬血清（SD0300）+4%FBS(SD0200)+2 mM/L-谷氨酰胺（SD0007)+1%G/A（SD0038）

完全培養(yǎng)基：RPMI1640完全培養(yǎng)基（MD0213)

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=7680068; DOI=10.1523/JNEUROSCI.13-03-01280.1993

Suri C., Fung B.P., Tischler A.S., Chikaraishi D.M.

Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice.

J. Neurosci. 13:1280-1291(1993)

Patent=US5591626

Chikaraishi D.M.

Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene.

Patent number US5591626, 07-Jan-1997

PubMed=9006967; DOI=10.1523/JNEUROSCI.17-04-01217.1997

Qi Y., Wang J.K.T., McMillian M., Chikaraishi D.M.

Characterization of a CNS cell line, CAD, in which morphological differentiation is initiated by serum deprivation.

J. Neurosci. 17:1217-1225(1997)