該細(xì)胞系由RAVanDeusen建立經(jīng)檢測(cè)，該細(xì)胞系未感染牛病毒性下痢病毒。    經(jīng)無(wú)支原體鑒定入庫(kù)。

動(dòng)物種別：貓

組織來(lái)源：腎，上皮細(xì)胞

形態(tài)：上皮細(xì)胞樣，貼壁生長(zhǎng)

培養(yǎng)基配方：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（MD0301）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=4130570; DOI=10.1007/BF02618435

Crandell R.A., Fabricant C.G., Nelson-Rees W.A.

Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK).

In Vitro 9:176-185(1973)

PubMed=9696876

Baumann J.G., Gunzburg W.H., Salmons B.

CrFK feline kidney cells produce an RD114-like endogenous virus that can package murine leukemia virus-based vectors.

J. Virol. 72:7685-7687(1998)

PubMed=20631117; DOI=10.1128/JCM.00992-10

Yoshikawa R., Sato E., Igarashi T., Miyazawa T.

Characterization of RD-114 virus isolated from a commercial canine vaccine manufactured using CRFK cells.

J. Clin. Microbiol. 48:3366-3369(2010)

PubMed=21029758; DOI=10.1016/j.virusres.2010.10.020

Okada M., Yoshikawa R., Shojima T., Baba K., Miyazawa T.

Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines.

Virus Res. 155:268-273(2011)

PubMed=23585909; DOI=10.1371/journal.pone.0061530

Fukuma A., Yoshikawa R., Miyazawa T., Yasuda J.

A new approach to establish a cell line with reduced risk of endogenous retroviruses.

PLoS ONE 8:E61530-E61530(2013)