RocketFuel? 無血清細胞培養(yǎng)基（動物組分）與我們的單批培養(yǎng)基和試劑保持一致。

該培養(yǎng)基為無血清培養(yǎng)基，培養(yǎng)基不含牛肝菌衍生物組分。但是RocketFuel?生長因子含有牛衍生的生長因子。想要獲得無動物源組分的補充，請參閱RocketFuel-R?。配方中規(guī)定了人源血清白蛋白和其他來自人重組蛋白成分。將8mlRocketFuel?細胞生長因子添加進500ml的基礎中即可使用。

規(guī)格：500ml基礎培養(yǎng)基，8mlRocketFuel?細胞生長因子，10ml Attachment Factor?細胞生長包被液.

RocketFuel? 無血清細胞培養(yǎng)基（動物組分）適用細胞：

被認證用于超過100個人源和動物源原代細胞和細胞系培養(yǎng)，包括:

人源:

所有ACBRI和CSC原代細胞

骨髓細胞(CFU-F和細胞爆裂培養(yǎng))

結(jié)締組織細胞

真皮成纖維細胞

內(nèi)皮細胞(主動脈、動脈、冠狀動脈、血管和靜脈)

胎兒肺細胞

胚胎和成體干細胞

成纖維細胞

腎小球系膜細胞

永生化/腫瘤來源間充質(zhì)細胞

微血管細胞(大腦、冠狀動脈、真皮、腎小球、肝、肺和視網(wǎng)膜)

平滑肌細胞

新生血管性腫瘤細胞

動物:

牛和豬大血管和微血管內(nèi)皮細胞

其他說明：

培養(yǎng)基中不添加激素。培養(yǎng)基不含抗生素; bacoff?(4Z0-643)是所有Cell Systems培養(yǎng)基的推薦抗生素。其MIC為或高于2 mg /mL。

培養(yǎng)基不含酚紅，諸多實驗表明細胞培養(yǎng)基中的酚紅與細胞表面的受體結(jié)合，使細胞產(chǎn)生反應，就像藥物水平的雌激素和/或炎癥介質(zhì)(如前列腺素或血栓素A2)存在于培養(yǎng)基中一樣。為了優(yōu)化研究人員的實驗條件，除非定制，正常的Cell Systems培養(yǎng)基都不含酚紅。

與 Attachment Factor?一起使用培養(yǎng)細胞效果更佳

為了達到更佳的細胞培養(yǎng)效果，促進細胞貼壁和新生能力，使用 RocketFuel? 無血清細胞培養(yǎng)基（動物組分）培養(yǎng)細胞，請按說明書的要求用Attachment Factor?包被培養(yǎng)瓶。

培養(yǎng)基保存：

基礎：2 - 8℃

8mlRocketFuel?細胞生長因子，10ml Attachment Factor?細胞生長包被液： -20℃。

SF-4Z0-500培養(yǎng)基由WFI配制，所有成分都符合cGMP和ISO標準。

聲明：產(chǎn)品僅用于科研。

"Galectin‐1 studies in proliferative diabetic retinopathy" Abu El-Esrar et al, Acta Ophthalmologica, 2019.

"Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage" Xiong et al. Scientific Reports, 2018.

"Association of HMGB1 with oxidative stress markers and regulators in PDR" Abu El-Asrar and Alam et al. Molecular Vision, 2017.

"Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells" Skottman et al, Experimental Cell Research, 2017.

"Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice" Shen et al PLoS One, 2016.

"Optimization of an in vitro human blood–brain barrier model: Application to blood monocyte transmigration assays"  Paradis et al. MethodsX, 2015.

"The combined role of galactose-deficient IgA1and streptococcal IgA-binding M protein in inducing IL-6 and C3 secretion from human mesangial cells: implications for IgA nephropathy" Schmitt et al. J Immunology, 2014.

"Ascorbic acid prevents high glucose-induced apoptosis in human brain pericytes" May et al BioChem Biophys Research Commun. 2014.

"S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis" El-Asrar et al Molecular Vision, 2014.

"Isolation of purified H and L polypeptide chains from guinea-pig gamma-2-immunoglobulin after mild reduction" Lamm et al, Immunology, 2014.

"Gas6 stimulates angiogenesis of human retinal endothelial cells and of zebrafish embryos via ERK1/2 signaling"  Kim et al. PLoS One, 2014.

"Synthesis and biological activity of NOSH-naproxen (AVT-219) and NOSH-sulindac (AVT-18A) as potent anti-inflammatory agents with chemotherapeutic potential"  Kodela et al, Med Chem Comm. 2013.

"Alterations of retinal vasculature in cystathionine-beta-synthase mutant mice, a model of hyperhomocysteinemia" Tawflik et al, IOVS, 2013.

"Prevention of VEGF-induced growth and tube formation in human retinal endothelial cell by aldose reductase inhibition"  Yadav et al, Journal of Diabetes, 2012.

"Localization of dichlorofluorescin in cardiac myocytes: implications for assessment of oxidative stress" Swift and Sarvazyan, Am J Physiol Heart Circ Physiol, 2011.

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