背景介紹/context：

Passage Reagent PRG-1 (EDTA-dPBS Solution 1X), 100ml.

A part of the Passage Reagent Group? (PRG).

規(guī)格：100ml

說(shuō)明：

Gain Consistency with our Single Lot Media and Reagents

The Passage Reagent Group? (PRG) is a matched set of CSC Certified? reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1? (EDTA-dPBS Solution), PRG-2? (Trypsin/EDTA-dPBS Solution) and PRG-3?(Trypsin Inhibitor-dPBS Solution) The chelating agent EDTA in PRG-1? prepares for PRG-2?, which contains highly purified trypsin. PRG-3? inactivates the protease in PRG-2? and stabilizes the cell membranes.

Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.

Intended for sequential use with PRG2? (Trypsin/EDTA-dPBS Solution) and PRG3? (Trypsin Inhibitor-dPBS solution).

Storage and Handling

Store at -20°C. Once opened, shelf life 30 days at +2 - 8°C

Cell Systems media and reagents are made with WFI, all components are cGMP and ISO Compliant, and are classified "Sterile".

"Optimization of an in vitro human blood–brain barrier model: Application to blood monocyte transmigration assays" Paradis et al. MethodsX, 2015

"S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis" Abu El-Asrar et al. Molecular Vision, 2014

"Isolation of purified H and L polypeptide chains from guinea-pig gamma-2-immunoglobulin after mild reduction" Lamm et al. Immunology, 2014)

"Optical Recording Reveals Novel Properties of GSK1016790A-Induced Vanilloid Transient Receptor Potential Channel TRPV4 Activity in Primary Human Endothelial Cells" Sullivan et al. Molecular Pharmacology, 2012

"High-Glucose-Induced Endothelial Cell Injury Is Inhibited by a Peptide Derived from Human Apolipoprotein E."  Bhattacharjee et al. PLOs One, 2012

"Soluble aggregates of the amyloid-beta are trapped by serum albumin to enhance amyloid-beta activation of endothelial cells" Barcelo et al. Journal of Biological Engineering, 2009

4Z0-610

SDS