此細(xì)胞株最初是為研究X染色體失活時(shí)提供X染色體，隨后發(fā)現(xiàn)它是極好的腎近曲小管上皮細(xì)胞的細(xì)胞培養(yǎng)模型。此細(xì)胞在培養(yǎng)時(shí)表達(dá)多種受體，并被廣泛地用作研究這些受體的模型。    經(jīng)無(wú)支原體鑒定入庫(kù)。

動(dòng)物種別：負(fù)鼠

組織來(lái)源：腎近曲小管，上皮細(xì)胞

形態(tài)：上皮細(xì)胞樣；貼壁生長(zhǎng)

培養(yǎng)基配方：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（MD0301）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=566717; DOI=10.1007/BF02616032

Koyama H., Goodpasture C., Miller M.M., Teplitz R.L., Riggs A.D.

Establishment and characterization of a cell line from the American opossum (Didelphys virginiana).

In Vitro 14:239-246(1978)

CLPUB00465

Esposito D.

Cytogenetic and mutagenic studies with an opossum cell line (Didelphys virginiana).

Thesis PhD (1982), Fordham University, United States

PubMed=6394469; DOI=10.1007/BF02619628

Keith D.H., Teplitz R.L., Riggs A.D.

Metaphase synchronization and chromosome preparation from the OK opossum cell line having a potentially isolatable X chromosome.

In Vitro 20:833-836(1984)

PubMed=1802405; DOI=10.1016/0309-1651(91)90094-Y

Courjault F., Gerin B., Leroy D., Chevalier J., Toutain H.

Morphological and biochemical characterization of the opossum kidney cell line and primary cultures of rabbit proximal tubule cells in serum-free defined medium.

Cell Biol. Int. Rep. 15:1225-1234(1991)

PubMed=28615248; DOI=10.1152/ajprenal.00228.2017

Eshbach M.L., Sethi R., Avula R., Lamb J., Hollingshead D.J., Finegold D.N., Locker J., Chandran U.R., Weisz O.A.

The transcriptome of the Didelphis virginiana opossum kidney OK proximal tubule cell line.

Am. J. Physiol. Renal Physiol. 313:F585-F595(2017)

PubMed=30379374; DOI=10.1113/EP087217

Guillen N., Caldas Y.A., Levi M., Sorribas V.

Identification and expression analysis of type II and type III Pi transporters in the opossum kidney cell line.

Exp. Physiol. 104:149-161(2019)