人膀胱移行細(xì)胞癌細(xì)胞T24來源于一名81歲白人女性。該移行細(xì)胞癌患者的白細(xì)胞和血清對T24細(xì)胞及相關(guān)細(xì)胞株有細(xì)胞毒性。該細(xì)胞倍增時(shí)間為19小時(shí)，含癌基因ras (H-ras)。

組織來源：膀胱

形態(tài)：上皮細(xì)胞樣，貼壁生長

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件: 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=864752; DOI=10.1093/jnci/58.6.1743

Marshall C.J., Franks L.M., Carbonell A.W.

Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.

J. Natl. Cancer Inst. 58:1743-1751(1977)

PubMed=870558; DOI=10.1177/25.4.870558

Benham F.J., Cottell D.C., Franks L.M., Wilson P.D.

Alkaline phosphatase activity in human bladder tumor cell lines.

J. Histochem. Cytochem. 25:266-274(1977)

PubMed=571047

Fogh J.

Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.

Natl. Cancer Inst. Monogr. 49:5-9(1978)

PubMed=651066; DOI=10.5980/jpnjurol1928.69.1_40

Kato T., Ishikawa K., Nemoto R.

Morphologic characterization of two established cell lines, T24 and MGH-U1, derived from human bladder carcinoma.

Nihon Hinyokika Gakkai Zasshi 69:40-46(1978)

PubMed=663932; DOI=10.1620/tjem.124.33

Kato T., Ishikawa K., Nemoto R., Senoo A., Amano Y.

Morphological characterization of two established cell lines, T24 and MGH-U1, derived from human urinary bladder carcinoma.

Tohoku J. Exp. Med. 124:339-349(1978)

PubMed=6244232

Williams R.D.

Human urologic cancer cell lines.

Invest. Urol. 17:359-363(1980)

PubMed=7017212; DOI=10.1093/jnci/66.6.1003

Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.

HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.

J. Natl. Cancer Inst. 66:1003-1012(1981)

PubMed=7185004; DOI=10.2302/kjm.31.127

Tachibana M.

Studies on cellular adhesiveness in five different culture cell lines derived from carcinoma of the urinary bladder.

Keio J. Med. 31:127-148(1982)