這株細(xì)胞是由BiedlerJL建立的兩株神經(jīng)組織來(lái)源的細(xì)胞中的一株（另一株是SK-N-SH），1971年9月分離得到。該細(xì)胞有中度的多巴胺-β-羥化酶活性，也有可用甲醛誘導(dǎo)熒光指示的細(xì)胞內(nèi)兒茶酚胺。  經(jīng)無(wú)支原體/STR鑒定入庫(kù)。

動(dòng)物種別：人

組織來(lái)源：神經(jīng)上皮瘤細(xì)胞

形態(tài)：上皮細(xì)胞樣， 貼壁生長(zhǎng)

培養(yǎng)基配方：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（MD0301）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：細(xì)胞凍存液Cell Freezing Medium（4Z0-705）

聲明：產(chǎn)品僅科研使用。

PubMed=4748425

Biedler J.L., Helson L., Spengler B.A.

Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.

Cancer Res. 33:2643-2652(1973)

DOI=10.1007/978-1-4757-1647-4_13

Biedler J.L.

Chromosome abnormalities in human tumor cells in culture.

(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=62055; DOI=10.1093/jnci/57.3.683

Biedler J.L., Spengler B.A.

A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.

J. Natl. Cancer Inst. 57:683-695(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221

Fogh J., Fogh J.M., Orfeo T.

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=29704

Biedler J.L., Roffler-Tarlov S., Schachner M., Freedman L.S.

Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones.

Cancer Res. 38:3751-3757(1978)

PubMed=7459858

Rousset M., Zweibaum A., Fogh J.

Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.

Cancer Res. 41:1165-1170(1981)

PubMed=7037175

Reynolds C.P., Reynolds D.A., Frenkel E.P., Smith R.G.

Selective toxicity of 6-hydroxydopamine and ascorbate for human neuroblastoma in vitro: a model for clearing marrow prior to autologous transplant.

Cancer Res. 42:1331-1336(1982)

PubMed=6582512; DOI=10.1073/pnas.81.2.568

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2987426; DOI=10.1007/BF00165170

Helson L., Helson C.

Human neuroblastoma cells and 13-cis-retinoic acid.

J. Neurooncol. 3:39-41(1985)

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.