A9細(xì)胞株由野生型細(xì)胞株L中分離得到。 對(duì)HAT選擇培養(yǎng)基敏感，并缺失了腺苷轉(zhuǎn)磷酸核糖基酶與次黃嘌呤轉(zhuǎn)磷酸核糖基酶 對(duì)8-氮鳥(niǎo)嘌呤(0.003mg/ml)和6-硫代鳥(niǎo)嘌呤(0.1mM)有抗性。    經(jīng)無(wú)支原體鑒定入庫(kù)。

動(dòng)物種別：小鼠

組織來(lái)源：皮下結(jié)締組織；疏松結(jié)締組織和脂肪；正常

形態(tài)：成纖維細(xì)胞樣,貼壁生長(zhǎng)

培養(yǎng)基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培養(yǎng)基（MD0101）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=14067106; DOI=10.1073/pnas.50.3.568

Littlefield J.W.

The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine.

Proc. Natl. Acad. Sci. U.S.A. 50:568-573(1963)

PubMed=14213660; DOI=10.1038/2031142a0

Littlefield J.W.

Three degrees of guanylic acid -- inosinic acid pyrophosphorylase deficiency in mouse fibroblasts.

Nature 203:1142-1144(1964)

PubMed=174085; DOI=10.1073/pnas.72.12.4971

Stiles C.D., Desmond W. Jr., Sato G., Saier M.H. Jr.

Failure of human cells transformed by simian virus 40 to form tumors in athymic nude mice.

Proc. Natl. Acad. Sci. U.S.A. 72:4971-4975(1975)

DOI=10.5962/bhl.title.4090

Coriell L.L., Greene A.E.

The human genetic mutant cell repository: list of genetic variants, chromosomal aberrations and normal cell cultures submitted to the repository. 4th edition. October 1977.

(In) Institute for Medical Research (Camden, N.J.); pp.1-171; National Institutes of Health; Bethesda (1977)

CLPUB00447

Mulivor R.A., Suchy S.F.

1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.

(In) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda (1992)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)