該細胞株經(jīng)過STR鑒定，與國際上ATCC、DSMZ、JCRB和RIKEN 公布的STR數(shù)據(jù)庫中8000余種細胞均不相符（匹配度低于80%），排除被常見細胞系污染的可能。由于Hth83細胞本身沒有公布可比對的STR圖譜數(shù)據(jù)，我們只能初步判斷該細胞可能為Hth83。STR結(jié)果如下：D5S818: 12；D13S317: 11,13；D7S820: 12；D16S539: 11,12；vWA: 16,19；THO1: 6,9；Amelogenin: X,Y；TPOX: 6,8；CSF1PO: 11。經(jīng)無支原體鑒定入庫。

動物種別：人

組織來源：甲狀腺癌細胞

形態(tài)：上皮細胞樣，貼壁生長

培養(yǎng)基配方：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：1640完全培養(yǎng)基（MD0201）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=10918212; DOI=10.1002/1096-9896(2000)9999:9999

Dahlman T., Lammerts E., Wik M., Bergstrom D., Grimelius L., Westermark K., Rubin K., Heldin N.-E.

Fibrosis in undifferentiated (anaplastic) thyroid carcinomas: evidence for a dual action of tumour cells in collagen type I synthesis.

J. Pathol. 191:376-386(2000)

PubMed=17465858; DOI=10.1089/thy.2006.0246

Lee J.J., Foukakis T., Hashemi J., Grimelius L., Heldin N.-E., Wallin G., Rudduck C., Lui W.-O., Hoog A., Larsson C.

Molecular cytogenetic profiles of novel and established human anaplastic thyroid carcinoma models.

Thyroid 17:289-301(2007)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690

Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.

Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.

Clin. Cancer Res. 17:7248-7264(2011)

PubMed=23833040; DOI=10.1210/jc.2013-2383

Landa I., Ganly I., Chan T.A., Mitsutake N., Matsuse M., Ibrahimpasic T., Ghossein R.A., Fagin J.A.

Frequent somatic TERT promoter mutations in thyroid cancer: higher prevalence in advanced forms of the disease.

J. Clin. Endocrinol. Metab. 98:E1562-E1566(2013)

PubMed=25365311; DOI=10.1210/jc.2014-2359

Garg M., Okamoto R., Nagata Y., Kanojia D., Venkatesan S., Anand M.T., Braunstein G.D., Said J.W., Doan N.B., Ho Q., Akagi T., Gery S., Liu L.-Z., Tan K.T., Chang W.J., Yang H., Ogawa S., Koeffler H.P.

Establishment and characterization of novel human primary and metastatic anaplastic thyroid cancer cell lines and their genomic evolution over a year as a primagraft.

J. Clin. Endocrinol. Metab. 100:725-735(2015)

PubMed=30737244; DOI=10.1158/1078-0432.CCR-18-2953

Landa I., Pozdeyev N., Korch C., Marlow L.A., Smallridge R.C., Copland J.A., Henderson Y.C., Lai S.Y., Clayman G.L., Onoda N., Tan A.C., Garcia-Rendueles M.E.R., Knauf J.A., Haugen B.R., Fagin J.A., Schweppe R.E.

Comprehensive genetic characterization of human thyroid cancer cell lines: a validated panel for preclinical studies.

Clin. Cancer Res. 25:3141-3151(2019)