MDA-MB-435S是一種紡錘形的細(xì)胞，1976年由其親本(435)中篩選得到。435是從31歲的轉(zhuǎn)移性乳腺導(dǎo)管腺癌女性患者胸水中分離得到。 當(dāng)用熒光染料對(duì)微管蛋白進(jìn)行染色時(shí)親本細(xì)胞顯現(xiàn)散布特征(II型)。 最近通過(guò)cDNA陣列研究表明，親本(MDA-MB-435)可歸入黑素瘤起源。 經(jīng)無(wú)支原體/STR鑒定入庫(kù)。

動(dòng)物種別：人

組織來(lái)源 ：黑素細(xì)胞，黑素瘤 以前認(rèn)為是乳腺; 乳房; 導(dǎo)管; 導(dǎo)管癌; 胸腺滲出液

形態(tài)：紡錘形，貼壁生長(zhǎng)

培養(yǎng)基配方：

90%L-15(MD1100)+10%胎牛血清(SD0200)+胰島素（SD0020）+G/A (SD0038)

完全培養(yǎng)基：L-15完全培養(yǎng)基（MD1104）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

DOI=10.1016/B978-0-12-333530-2.50009-5

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